Impembelelo ye-Anticancer ye-Ganoderma lucidum kwiiSeli ze-Osteosarcoma yoMntu

Uphononongo lwethu lubonisa ukuba i-Ganoderma lucidum/reishi/lingzhi ibonisa iipropathi ze-antitumor kwiiseli ze-osteosarcoma kwi-vitro.Kwafunyaniswa ukuba i-Ganoderma lucidum inqanda ukukhula kweeseli zomhlaza webele kunye nokufuduka ngokucinezela umqondiso we-Wnt / β-catenin.Icinezela umhlaza wemiphunga ngokuphazamiseka kokunamathela kunye nokungeniswa kwe-MDM2-mediated Slug degradation.I-Ganoderma lucidum inqanda umhlaza webele ngokuthoba indlela ye-PI3K/AKT/mTOR, i-Ganoderma lucidum idlala indima ye-antitumor kwiiseli ezibukhali ze-leukemia ngokuvala indlela ye-MAPK.

I-CCK-8 kunye novavanyo lokuqulunqwa kwekholoni, ukuvavanya umphumo weGanoderma lucidum kwi-osteosarcoma cell line viability kunye nokwanda, ibonise ukuba i-Ganoderma lucidum icinezela ukwanda kweeseli ze-MG63 kunye ne-U2-OS ngexesha kunye nokuxhomekeka koxinzelelo, kwaye iyanciphisa ukukwazi kweeseli ukwenza ikoloni.

I-Ganoderma lucidum iphakamisa ukubonakaliswa kweejene ze-proapoptotic, kwaye uhlalutyo lwe-cytometry lubonisa ukuba i-apoptosis ye-MG63 kunye neeseli ze-U2-OS zanda emva kokuba unyango lwe-Ganoderma lucidum.Ukufuduka kweeseli sisiseko seendlela ezahlukeneyo zokuziphatha kwebhayoloji, kubandakanya i-angiogenesis, ukuphiliswa kwenxeba, ukudumba, kunye ne-metastasis yomhlaza.I-Ganoderma lucidum inciphisa ukufuduka kunye nokuhlaselwa kwayo yomibini imigca yeeseli kwaye inqanda ukwanda, ukufuduka, kunye nokuhlasela, kwaye yenza i-apoptosis yeeseli ze-osteosarcoma.

Ukubonakaliswa kwe-Aberrant Wnt / β-catenin kuhambelana ngokusondeleyo nokubunjwa, i-metastasis, kunye ne-apoptosis yeentlobo ezininzi ze-cancer, kunye nokulawulwa kwe-Wnt / β-catenin ukubonakaliswa kubonwa kwi-osteosarcoma.

Kulo pho nonongo, i-dual-luciferase i-reporter assays ibonise ukuba i-Ganoderma lucidum yonyango ibhlokhi i-CHIR-99021-activated Wnt / β-catenin signaling.Oku kuphinda kubonakaliswe ngokubonisa kwethu ukuba ushicilelo lwejene ezijoliswe kuyo ze-Wnt, ezifana ne-LRP5, i-β-catenin, i-cyclin D1, kunye ne-MMP-9, ivinjelwe xa iiseli ze-osteosarcoma ziphathwa nge-Ganoderma lucidum.

Uphononongo lwangaphambili lubonise kwiisampulu zeklinikhi ukuba i-LRP5 ilawulwa kwi-osteosarcoma ngokumalunga nezicubu eziqhelekileyo, kunye nokubonakaliswa kwe-LRP5 ehambelana nesifo se-metastatic kunye nokusinda okungenasifo esibi, okwenza i-LRP5 ibe yinto ekujoliswe kuyo yonyango ye-osteosarcoma.

I-β-catenin ngokwayo iyinjongo ephambili kwindlela yokubonisa i-Wnt / β-catenin, kunye nokubonakaliswa kwe-β-catenin kwi-osteosarcoma kwanda kakhulu.Xa i-β-catenin itshintshela kwi-nucleus esuka kwi-cytoplasm, ivuselela ukubonakaliswa kweejene ezijoliswe kuyo ezantsi, ezibandakanya i-cyclin D1, i-C-Myc, kunye ne-MMPs.

I-Myc yenye ye-proto-oncogenes enkulu kwaye idlala indima ebalulekileyo ekulawuleni ukusebenza, ukubhaliswa, kunye nokuvinjelwa kwe-gene expression.Kuye kwaxelwa ukuba ukunyanzeliswa kwe-C-Myc oncogene kubangela ukuguga kunye ne-apoptosis yeentlobo ezininzi ze-tumor cell, kubandakanywa. i-osteosarcoma.

I-Cyclin D1 ngumjikelezo weseli obalulekileyo we-G1 umlawuli wesigaba kwaye ukhawuleza i-G1 / S yenguqu yesigaba.Ukubonakaliswa okugqithisileyo kwe-cyclin D1 kunokunciphisa umjikelo weseli kwaye kukhuthaze ukwanda ngokukhawuleza kweeseli kwiindidi zamathumba.

I-MMP-2 kunye ne-MMP-9 zi-stromelysins ezikwaziyo ukuthobisa amacandelo e-matrix e-extracellular, uphawu olubalulekileyo lwe-angiogenesis ye-tumor kunye nokuhlasela.

Oku kuphakamisa ukuba i-Wnt / β-catenin i-genenes ekujoliswe kuyo idlala indima ephambili ekuqhubeleni phambili kwe-osteosarcoma, kwaye ukuvala ezi nodi zesignali kunokuba nefuthe elimangalisayo lonyango.

Emva koko, siye safumanisa ukubonakaliswa kwe-mRNA kunye neprotheni ye-Wnt / β-catenin ehambelana nomqondiso we-genenes ekujoliswe kuyo yi-PCR kunye ne-western blotting.Kuyo yomibini imigca yeeseli, iGanoderma lucidum inqanda ukubonakaliswa kwezi proteni kunye nofuzo.Ezi ziphumo zibonisa ngakumbi ukuba i-Ganoderma lucidum inqanda ukubonakaliswa kwe-Wnt/β-catenin ngokujolisa kwi-LRP5, β-catenin, C-Myc, cyclin D1, MMP-2, kunye ne-MMP-9.

I-E-cadherin yi-transmembrane glycoprotein echazwe ngokubanzi kwiiseli ze-epithelial kwaye idibanisa ukunamathela phakathi kweeseli ze-epithelial kunye neeseli ze-stromal.Ukususwa okanye ukulahleka kwe-E-cadherin yokubonakalisa kukhokelela ekulahlekeni okanye ekunciphiseni ukunamathela phakathi kweeseli zethumba, okwenza iiseli zethumba ukuba zihambe ngokulula, kwaye emva koko zenze ithumba lingene, lisasazeke, kwaye libe ne-metastasize.Kule sifundo, sifumene ukuba i-Ganoderma lucidum inokunyusa i-E-cadherin, ngaloo ndlela iphikisana ne-Wnt / β-catenin-mediated phenotype yeeseli ze-osteosarcoma.

Ukuqukumbela, iziphumo zethu zibonisa ukuba i-Ganoderma lucidum ivimba i-osteosarcoma Wnt / β-catenin yokubonisa kwaye ekugqibeleni ikhokelela ekunciphiseni komsebenzi weseli ye-osteosarcoma.Ezi ziphumo zibonisa ukuba i-Ganoderma lucidum inokuba luncedo kwaye i-arhente yonyango esebenzayo kunyango lwe-osteosarcoma, iimveliso ezinxulumene nazo zibandakanya.ganoderma lucidum spore oil softgels/reishi spore oiii-softgels,ganoderma lucidum spore powder/reishi spore powder